Oxygen imaging of hypoxic pockets in the mouse cerebral cortex

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Oxygen imaging of hypoxic pockets in the mouse cerebral cortex. / Beinlich, Felix R M; Asiminas, Antonios; Untiet, Verena; Bojarowska, Zuzanna; Plá, Virginia; Sigurdsson, Björn; Timmel, Vincenzo; Gehrig, Lukas; Graber, Michael H; Hirase, Hajime; Nedergaard, Maiken.

In: Science (New York, N.Y.), Vol. 383, No. 6690, 2024, p. 1471-1478.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Beinlich, FRM, Asiminas, A, Untiet, V, Bojarowska, Z, Plá, V, Sigurdsson, B, Timmel, V, Gehrig, L, Graber, MH, Hirase, H & Nedergaard, M 2024, 'Oxygen imaging of hypoxic pockets in the mouse cerebral cortex', Science (New York, N.Y.), vol. 383, no. 6690, pp. 1471-1478. https://doi.org/10.1126/science.adn1011

APA

Beinlich, F. R. M., Asiminas, A., Untiet, V., Bojarowska, Z., Plá, V., Sigurdsson, B., Timmel, V., Gehrig, L., Graber, M. H., Hirase, H., & Nedergaard, M. (2024). Oxygen imaging of hypoxic pockets in the mouse cerebral cortex. Science (New York, N.Y.), 383(6690), 1471-1478. https://doi.org/10.1126/science.adn1011

Vancouver

Beinlich FRM, Asiminas A, Untiet V, Bojarowska Z, Plá V, Sigurdsson B et al. Oxygen imaging of hypoxic pockets in the mouse cerebral cortex. Science (New York, N.Y.). 2024;383(6690):1471-1478. https://doi.org/10.1126/science.adn1011

Author

Beinlich, Felix R M ; Asiminas, Antonios ; Untiet, Verena ; Bojarowska, Zuzanna ; Plá, Virginia ; Sigurdsson, Björn ; Timmel, Vincenzo ; Gehrig, Lukas ; Graber, Michael H ; Hirase, Hajime ; Nedergaard, Maiken. / Oxygen imaging of hypoxic pockets in the mouse cerebral cortex. In: Science (New York, N.Y.). 2024 ; Vol. 383, No. 6690. pp. 1471-1478.

Bibtex

@article{dfc4de0a7fda4cb49c885e676c23cfb6,
title = "Oxygen imaging of hypoxic pockets in the mouse cerebral cortex",
abstract = "Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po2) dynamics under physiological conditions. Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po2 imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined {"}hypoxic pockets{"} and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.",
keywords = "Mice, Animals, Oxygen, Brain/blood supply, Consciousness, Diagnostic Imaging, Cerebral Cortex/diagnostic imaging",
author = "Beinlich, {Felix R M} and Antonios Asiminas and Verena Untiet and Zuzanna Bojarowska and Virginia Pl{\'a} and Bj{\"o}rn Sigurdsson and Vincenzo Timmel and Lukas Gehrig and Graber, {Michael H} and Hajime Hirase and Maiken Nedergaard",
year = "2024",
doi = "10.1126/science.adn1011",
language = "English",
volume = "383",
pages = "1471--1478",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6690",

}

RIS

TY - JOUR

T1 - Oxygen imaging of hypoxic pockets in the mouse cerebral cortex

AU - Beinlich, Felix R M

AU - Asiminas, Antonios

AU - Untiet, Verena

AU - Bojarowska, Zuzanna

AU - Plá, Virginia

AU - Sigurdsson, Björn

AU - Timmel, Vincenzo

AU - Gehrig, Lukas

AU - Graber, Michael H

AU - Hirase, Hajime

AU - Nedergaard, Maiken

PY - 2024

Y1 - 2024

N2 - Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po2) dynamics under physiological conditions. Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po2 imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined "hypoxic pockets" and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.

AB - Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po2) dynamics under physiological conditions. Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po2 imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined "hypoxic pockets" and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.

KW - Mice

KW - Animals

KW - Oxygen

KW - Brain/blood supply

KW - Consciousness

KW - Diagnostic Imaging

KW - Cerebral Cortex/diagnostic imaging

U2 - 10.1126/science.adn1011

DO - 10.1126/science.adn1011

M3 - Journal article

C2 - 38547288

VL - 383

SP - 1471

EP - 1478

JO - Science

JF - Science

SN - 0036-8075

IS - 6690

ER -

ID: 387028899