Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
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Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.
Original language | English |
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Article number | 102041 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 1 |
Number of pages | 18 |
ISSN | 2666-1667 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
- Rats, Animals, Brain/diagnostic imaging, Microscopy, Confocal
Research areas
ID: 371030782