Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Research output: Contribution to journal › Journal article › Research › peer-review

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Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices. / Giacomoni, Jessica; Habekost, Mette; Cepeda-Prado, Efrain; Mattsson, Bengt; Ottosson, Daniella Rylander; Parmar, Malin; Kajtez, Janko.

In: STAR Protocols, Vol. 4, No. 1, 102041, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Giacomoni, J, Habekost, M, Cepeda-Prado, E, Mattsson, B, Ottosson, DR, Parmar, M & Kajtez, J 2023, 'Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices', STAR Protocols, vol. 4, no. 1, 102041. https://doi.org/10.1016/j.xpro.2022.102041

APA

Giacomoni, J., Habekost, M., Cepeda-Prado, E., Mattsson, B., Ottosson, D. R., Parmar, M., & Kajtez, J. (2023). Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices. STAR Protocols, 4(1), [102041]. https://doi.org/10.1016/j.xpro.2022.102041

Vancouver

Giacomoni J, Habekost M, Cepeda-Prado E, Mattsson B, Ottosson DR, Parmar M et al. Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices. STAR Protocols. 2023;4(1). 102041. https://doi.org/10.1016/j.xpro.2022.102041

Author

Giacomoni, Jessica ; Habekost, Mette ; Cepeda-Prado, Efrain ; Mattsson, Bengt ; Ottosson, Daniella Rylander ; Parmar, Malin ; Kajtez, Janko. / Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices. In: STAR Protocols. 2023 ; Vol. 4, No. 1.

Bibtex

@article{f52ebdcb37c54438a203de22a97cbba8,
title = "Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices",
abstract = "Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.",
keywords = "Rats, Animals, Brain/diagnostic imaging, Microscopy, Confocal",
author = "Jessica Giacomoni and Mette Habekost and Efrain Cepeda-Prado and Bengt Mattsson and Ottosson, {Daniella Rylander} and Malin Parmar and Janko Kajtez",
note = "Copyright {\textcopyright} 2022 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2023",
doi = "10.1016/j.xpro.2022.102041",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

AU - Giacomoni, Jessica

AU - Habekost, Mette

AU - Cepeda-Prado, Efrain

AU - Mattsson, Bengt

AU - Ottosson, Daniella Rylander

AU - Parmar, Malin

AU - Kajtez, Janko

N1 - Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2023

Y1 - 2023

N2 - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

AB - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

KW - Rats

KW - Animals

KW - Brain/diagnostic imaging

KW - Microscopy, Confocal

U2 - 10.1016/j.xpro.2022.102041

DO - 10.1016/j.xpro.2022.102041

M3 - Journal article

C2 - 36853668

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 1

M1 - 102041

ER -

ID: 371030782