Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep
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Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.
Original language | English |
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Article number | e4734 |
Journal | Bio-protocol |
Volume | 13 |
Issue number | 15 |
Number of pages | 17 |
ISSN | 2331-8325 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Publisher Copyright:
Copyright: © 2023 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
- Biosensors, EEG, Fiber photometry, Fluorescence, Locus coeruleus, Noradrenaline, Sleep
Research areas
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