Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep. / Andersen, Mie; Tsopanidou, Anastasia; Radovanovic, Tessa; Compere, Viviane Noelani; Hauglund, Natalie; Nedergaard, Maiken; Kjaerby, Celia.

In: Bio-protocol, Vol. 13, No. 15, e4734, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersen, M, Tsopanidou, A, Radovanovic, T, Compere, VN, Hauglund, N, Nedergaard, M & Kjaerby, C 2023, 'Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep', Bio-protocol, vol. 13, no. 15, e4734. https://doi.org/10.21769/BioProtoc.4734

APA

Andersen, M., Tsopanidou, A., Radovanovic, T., Compere, V. N., Hauglund, N., Nedergaard, M., & Kjaerby, C. (2023). Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep. Bio-protocol, 13(15), [e4734]. https://doi.org/10.21769/BioProtoc.4734

Vancouver

Andersen M, Tsopanidou A, Radovanovic T, Compere VN, Hauglund N, Nedergaard M et al. Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep. Bio-protocol. 2023;13(15). e4734. https://doi.org/10.21769/BioProtoc.4734

Author

Andersen, Mie ; Tsopanidou, Anastasia ; Radovanovic, Tessa ; Compere, Viviane Noelani ; Hauglund, Natalie ; Nedergaard, Maiken ; Kjaerby, Celia. / Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep. In: Bio-protocol. 2023 ; Vol. 13, No. 15.

Bibtex

@article{2e5aa6f5ad794a6c8ddcad8c0b52ed51,
title = "Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep",
abstract = "Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.",
keywords = "Biosensors, EEG, Fiber photometry, Fluorescence, Locus coeruleus, Noradrenaline, Sleep",
author = "Mie Andersen and Anastasia Tsopanidou and Tessa Radovanovic and Compere, {Viviane Noelani} and Natalie Hauglund and Maiken Nedergaard and Celia Kjaerby",
note = "Publisher Copyright: Copyright: {\textcopyright} 2023 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).",
year = "2023",
doi = "10.21769/BioProtoc.4734",
language = "English",
volume = "13",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "15",

}

RIS

TY - JOUR

T1 - Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep

AU - Andersen, Mie

AU - Tsopanidou, Anastasia

AU - Radovanovic, Tessa

AU - Compere, Viviane Noelani

AU - Hauglund, Natalie

AU - Nedergaard, Maiken

AU - Kjaerby, Celia

N1 - Publisher Copyright: Copyright: © 2023 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).

PY - 2023

Y1 - 2023

N2 - Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.

AB - Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.

KW - Biosensors

KW - EEG

KW - Fiber photometry

KW - Fluorescence

KW - Locus coeruleus

KW - Noradrenaline

KW - Sleep

U2 - 10.21769/BioProtoc.4734

DO - 10.21769/BioProtoc.4734

M3 - Journal article

C2 - 37575397

AN - SCOPUS:85169554318

VL - 13

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 15

M1 - e4734

ER -

ID: 371283981