The state of brain activity modulates cerebrospinal fluid transport
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The state of brain activity modulates cerebrospinal fluid transport. / Miyakoshi, Leo M.; Stæger, Frederik F.; Li, Qianliang; Pan, Chenchen; Xie, Lulu; Kang, Hongyi; Pavan, Chiara; Dang, Juliana; Sun, Qian; Ertürk, Ali; Nedergaard, Maiken.
In: Progress in Neurobiology, Vol. 229, 102512, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The state of brain activity modulates cerebrospinal fluid transport
AU - Miyakoshi, Leo M.
AU - Stæger, Frederik F.
AU - Li, Qianliang
AU - Pan, Chenchen
AU - Xie, Lulu
AU - Kang, Hongyi
AU - Pavan, Chiara
AU - Dang, Juliana
AU - Sun, Qian
AU - Ertürk, Ali
AU - Nedergaard, Maiken
N1 - Publisher Copyright: © 2023
PY - 2023
Y1 - 2023
N2 - Earlier studies based on 2-photon imaging have shown that glymphatic cerebrospinal fluid (CSF) transport is regulated by the sleep-wake cycle. To examine this association, we used 3DISCO whole-body tissue clearing to map CSF tracer distribution in awake, sleeping and ketamine-xylazine anesthetized mice. The results of our analysis showed that CSF tracers entered the brain to a significantly larger extent in natural sleep or ketamine-xylazine anesthesia than in wakefulness. Furthermore, awake mice showed preferential transport of CSF tracers in the rostro-caudal direction towards the cervical and spinal cord lymphatic vessels, and hence to venous circulation and excretion by the kidneys. The study extends the current literature by showing that CSF dynamics on the whole-body scale is controlled by the state of brain activity.
AB - Earlier studies based on 2-photon imaging have shown that glymphatic cerebrospinal fluid (CSF) transport is regulated by the sleep-wake cycle. To examine this association, we used 3DISCO whole-body tissue clearing to map CSF tracer distribution in awake, sleeping and ketamine-xylazine anesthetized mice. The results of our analysis showed that CSF tracers entered the brain to a significantly larger extent in natural sleep or ketamine-xylazine anesthesia than in wakefulness. Furthermore, awake mice showed preferential transport of CSF tracers in the rostro-caudal direction towards the cervical and spinal cord lymphatic vessels, and hence to venous circulation and excretion by the kidneys. The study extends the current literature by showing that CSF dynamics on the whole-body scale is controlled by the state of brain activity.
KW - Cerebrospinal fluid
KW - Neurodegeneration
KW - Neuromodulators
KW - Perivascular spaces
KW - Sleep
KW - Tissue clearance
U2 - 10.1016/j.pneurobio.2023.102512
DO - 10.1016/j.pneurobio.2023.102512
M3 - Journal article
C2 - 37482196
AN - SCOPUS:85166213773
VL - 229
JO - Progress in Neurobiology
JF - Progress in Neurobiology
SN - 0301-0082
M1 - 102512
ER -
ID: 371287343