Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging

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Standard

Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging. / Esmail, Tina; Nedergaard, Maiken; Beinlich, Felix R.M.

In: STAR Protocols, Vol. 4, No. 4, 102652, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Esmail, T, Nedergaard, M & Beinlich, FRM 2023, 'Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging', STAR Protocols, vol. 4, no. 4, 102652. https://doi.org/10.1016/j.xpro.2023.102652

APA

Esmail, T., Nedergaard, M., & Beinlich, F. R. M. (2023). Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging. STAR Protocols, 4(4), [102652]. https://doi.org/10.1016/j.xpro.2023.102652

Vancouver

Esmail T, Nedergaard M, Beinlich FRM. Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging. STAR Protocols. 2023;4(4). 102652. https://doi.org/10.1016/j.xpro.2023.102652

Author

Esmail, Tina ; Nedergaard, Maiken ; Beinlich, Felix R.M. / Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging. In: STAR Protocols. 2023 ; Vol. 4, No. 4.

Bibtex

@article{5c5d198a06104b52a8a77b8d1e4bf632,
title = "Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging",
abstract = "In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to M{\o}llg{\aa}rd et al. (2023).1",
keywords = "Health Sciences, Microscopy, Neuroscience",
author = "Tina Esmail and Maiken Nedergaard and Beinlich, {Felix R.M.}",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
doi = "10.1016/j.xpro.2023.102652",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging

AU - Esmail, Tina

AU - Nedergaard, Maiken

AU - Beinlich, Felix R.M.

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2023

Y1 - 2023

N2 - In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1

AB - In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1

KW - Health Sciences

KW - Microscopy

KW - Neuroscience

U2 - 10.1016/j.xpro.2023.102652

DO - 10.1016/j.xpro.2023.102652

M3 - Journal article

C2 - 37883226

AN - SCOPUS:85174721969

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 4

M1 - 102652

ER -

ID: 371926664