Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo. / Dubanet, Olivier; Da Silva, Arnaldo Ferreira Gomes; Frick, Andreas; Hirase, Hajime; Beyeler, Anna; Leinekugel, Xavier.

In: Cell Reports, Vol. 36, No. 2, 109381, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dubanet, O, Da Silva, AFG, Frick, A, Hirase, H, Beyeler, A & Leinekugel, X 2021, 'Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo', Cell Reports, vol. 36, no. 2, 109381. https://doi.org/10.1016/j.celrep.2021.109381

APA

Dubanet, O., Da Silva, A. F. G., Frick, A., Hirase, H., Beyeler, A., & Leinekugel, X. (2021). Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo. Cell Reports, 36(2), [109381]. https://doi.org/10.1016/j.celrep.2021.109381

Vancouver

Dubanet O, Da Silva AFG, Frick A, Hirase H, Beyeler A, Leinekugel X. Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo. Cell Reports. 2021;36(2). 109381. https://doi.org/10.1016/j.celrep.2021.109381

Author

Dubanet, Olivier ; Da Silva, Arnaldo Ferreira Gomes ; Frick, Andreas ; Hirase, Hajime ; Beyeler, Anna ; Leinekugel, Xavier. / Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo. In: Cell Reports. 2021 ; Vol. 36, No. 2.

Bibtex

@article{93e786e1e3be4adb8d7fdadbe942413a,
title = "Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo",
abstract = "The hypothesis that reversed, excitatory GABA may be involved in various brain pathologies, including epi-leptogenesis, is appealing but controversial because of the technical difficulty of probing endogenous GABAergic synaptic function in vivo. We overcome this challenge by non-invasive extracellular recording of neuronal firing responses to optogenetically evoked and spontaneously occurring inhibitory perisomatic GABAergic field potentials, generated by individual parvalbumin interneurons on their target pyramidal cells. Our direct probing of GABAergic transmission suggests a rather anecdotal participation of excitatory GABA in two specificmodels of epileptogenesis in the mouse CA3 circuit in vivo, even though this does not preclude its expression in other brain areas or pathological conditions. Our approach allows the detection of distinct alterations of inhibition during spontaneous activity in vivo, with high sensitivity. It represents a promising tool for the investigation of excitatory GABA in different pathological conditions that may affect the hippocampal circuit.",
keywords = "CATION-CHLORIDE COTRANSPORTERS, TEMPORAL-LOBE EPILEPSY, SHARP-WAVE-RIPPLES, HIPPOCAMPAL SCLEROSIS, EXCITATORY ACTIONS, GABA, INTERNEURONS, INHIBITION, EXCITABILITY, CELLS",
author = "Olivier Dubanet and {Da Silva}, {Arnaldo Ferreira Gomes} and Andreas Frick and Hajime Hirase and Anna Beyeler and Xavier Leinekugel",
year = "2021",
doi = "10.1016/j.celrep.2021.109381",
language = "English",
volume = "36",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Probing the polarity of spontaneous perisomatic GABAergic synaptic transmission in the mouse CA3 circuit in vivo

AU - Dubanet, Olivier

AU - Da Silva, Arnaldo Ferreira Gomes

AU - Frick, Andreas

AU - Hirase, Hajime

AU - Beyeler, Anna

AU - Leinekugel, Xavier

PY - 2021

Y1 - 2021

N2 - The hypothesis that reversed, excitatory GABA may be involved in various brain pathologies, including epi-leptogenesis, is appealing but controversial because of the technical difficulty of probing endogenous GABAergic synaptic function in vivo. We overcome this challenge by non-invasive extracellular recording of neuronal firing responses to optogenetically evoked and spontaneously occurring inhibitory perisomatic GABAergic field potentials, generated by individual parvalbumin interneurons on their target pyramidal cells. Our direct probing of GABAergic transmission suggests a rather anecdotal participation of excitatory GABA in two specificmodels of epileptogenesis in the mouse CA3 circuit in vivo, even though this does not preclude its expression in other brain areas or pathological conditions. Our approach allows the detection of distinct alterations of inhibition during spontaneous activity in vivo, with high sensitivity. It represents a promising tool for the investigation of excitatory GABA in different pathological conditions that may affect the hippocampal circuit.

AB - The hypothesis that reversed, excitatory GABA may be involved in various brain pathologies, including epi-leptogenesis, is appealing but controversial because of the technical difficulty of probing endogenous GABAergic synaptic function in vivo. We overcome this challenge by non-invasive extracellular recording of neuronal firing responses to optogenetically evoked and spontaneously occurring inhibitory perisomatic GABAergic field potentials, generated by individual parvalbumin interneurons on their target pyramidal cells. Our direct probing of GABAergic transmission suggests a rather anecdotal participation of excitatory GABA in two specificmodels of epileptogenesis in the mouse CA3 circuit in vivo, even though this does not preclude its expression in other brain areas or pathological conditions. Our approach allows the detection of distinct alterations of inhibition during spontaneous activity in vivo, with high sensitivity. It represents a promising tool for the investigation of excitatory GABA in different pathological conditions that may affect the hippocampal circuit.

KW - CATION-CHLORIDE COTRANSPORTERS

KW - TEMPORAL-LOBE EPILEPSY

KW - SHARP-WAVE-RIPPLES

KW - HIPPOCAMPAL SCLEROSIS

KW - EXCITATORY ACTIONS

KW - GABA

KW - INTERNEURONS

KW - INHIBITION

KW - EXCITABILITY

KW - CELLS

U2 - 10.1016/j.celrep.2021.109381

DO - 10.1016/j.celrep.2021.109381

M3 - Journal article

C2 - 34260906

VL - 36

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 2

M1 - 109381

ER -

ID: 274665032