Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. / Wang, Xiaowen; Delle, Christine; Asiminas, Antonis; Akther, Sonam; Vittani, Marta; Brøgger, Peter; Kusk, Peter; Vo, Camilla Trang; Radovanovic, Tessa; Konno, Ayumu; Hirai, Hirokazu; Fukuda, Masahiro; Weikop, Pia; Goldman, Steven A; Nedergaard, Maiken; Hirase, Hajime.

In: Cell Reports Methods, Vol. 2, No. 10, 100302, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wang, X, Delle, C, Asiminas, A, Akther, S, Vittani, M, Brøgger, P, Kusk, P, Vo, CT, Radovanovic, T, Konno, A, Hirai, H, Fukuda, M, Weikop, P, Goldman, SA, Nedergaard, M & Hirase, H 2022, 'Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation', Cell Reports Methods, vol. 2, no. 10, 100302. https://doi.org/10.1016/j.crmeth.2022.100302

APA

Wang, X., Delle, C., Asiminas, A., Akther, S., Vittani, M., Brøgger, P., Kusk, P., Vo, C. T., Radovanovic, T., Konno, A., Hirai, H., Fukuda, M., Weikop, P., Goldman, S. A., Nedergaard, M., & Hirase, H. (2022). Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Cell Reports Methods, 2(10), [100302]. https://doi.org/10.1016/j.crmeth.2022.100302

Vancouver

Wang X, Delle C, Asiminas A, Akther S, Vittani M, Brøgger P et al. Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Cell Reports Methods. 2022;2(10). 100302. https://doi.org/10.1016/j.crmeth.2022.100302

Author

Wang, Xiaowen ; Delle, Christine ; Asiminas, Antonis ; Akther, Sonam ; Vittani, Marta ; Brøgger, Peter ; Kusk, Peter ; Vo, Camilla Trang ; Radovanovic, Tessa ; Konno, Ayumu ; Hirai, Hirokazu ; Fukuda, Masahiro ; Weikop, Pia ; Goldman, Steven A ; Nedergaard, Maiken ; Hirase, Hajime. / Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. In: Cell Reports Methods. 2022 ; Vol. 2, No. 10.

Bibtex

@article{db90b820d0ce43a3a8ec4599e9e957b4,
title = "Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation",
abstract = "Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.",
author = "Xiaowen Wang and Christine Delle and Antonis Asiminas and Sonam Akther and Marta Vittani and Peter Br{\o}gger and Peter Kusk and Vo, {Camilla Trang} and Tessa Radovanovic and Ayumu Konno and Hirokazu Hirai and Masahiro Fukuda and Pia Weikop and Goldman, {Steven A} and Maiken Nedergaard and Hajime Hirase",
note = "{\textcopyright} 2022 The Author(s).",
year = "2022",
doi = "10.1016/j.crmeth.2022.100302",
language = "English",
volume = "2",
journal = "Cell Reports Methods",
issn = "2667-2375",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation

AU - Wang, Xiaowen

AU - Delle, Christine

AU - Asiminas, Antonis

AU - Akther, Sonam

AU - Vittani, Marta

AU - Brøgger, Peter

AU - Kusk, Peter

AU - Vo, Camilla Trang

AU - Radovanovic, Tessa

AU - Konno, Ayumu

AU - Hirai, Hirokazu

AU - Fukuda, Masahiro

AU - Weikop, Pia

AU - Goldman, Steven A

AU - Nedergaard, Maiken

AU - Hirase, Hajime

N1 - © 2022 The Author(s).

PY - 2022

Y1 - 2022

N2 - Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.

AB - Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.

U2 - 10.1016/j.crmeth.2022.100302

DO - 10.1016/j.crmeth.2022.100302

M3 - Journal article

C2 - 36313804

VL - 2

JO - Cell Reports Methods

JF - Cell Reports Methods

SN - 2667-2375

IS - 10

M1 - 100302

ER -

ID: 329708257