Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. / Nasu, Yusuke; Aggarwal, Abhi; Le, Giang N.T.; Vo, Camilla Trang; Kambe, Yuki; Wang, Xinxing; Beinlich, Felix R.M.; Lee, Ashley Bomin; Ram, Tina R.; Wang, Fangying; Gorzo, Kelsea A.; Kamijo, Yuki; Boisvert, Marc; Nishinami, Suguru; Kawamura, Genki; Ozawa, Takeaki; Toda, Hirofumi; Gordon, Grant R.; Ge, Shaoyu; Hirase, Hajime; Nedergaard, Maiken; Paquet, Marie Eve; Drobizhev, Mikhail; Podgorski, Kaspar; Campbell, Robert E.

In: Nature Communications, Vol. 14, No. 1, 6598, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nasu, Y, Aggarwal, A, Le, GNT, Vo, CT, Kambe, Y, Wang, X, Beinlich, FRM, Lee, AB, Ram, TR, Wang, F, Gorzo, KA, Kamijo, Y, Boisvert, M, Nishinami, S, Kawamura, G, Ozawa, T, Toda, H, Gordon, GR, Ge, S, Hirase, H, Nedergaard, M, Paquet, ME, Drobizhev, M, Podgorski, K & Campbell, RE 2023, 'Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging', Nature Communications, vol. 14, no. 1, 6598. https://doi.org/10.1038/s41467-023-42230-5

APA

Nasu, Y., Aggarwal, A., Le, G. N. T., Vo, C. T., Kambe, Y., Wang, X., Beinlich, F. R. M., Lee, A. B., Ram, T. R., Wang, F., Gorzo, K. A., Kamijo, Y., Boisvert, M., Nishinami, S., Kawamura, G., Ozawa, T., Toda, H., Gordon, G. R., Ge, S., ... Campbell, R. E. (2023). Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. Nature Communications, 14(1), [6598]. https://doi.org/10.1038/s41467-023-42230-5

Vancouver

Nasu Y, Aggarwal A, Le GNT, Vo CT, Kambe Y, Wang X et al. Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. Nature Communications. 2023;14(1). 6598. https://doi.org/10.1038/s41467-023-42230-5

Author

Nasu, Yusuke ; Aggarwal, Abhi ; Le, Giang N.T. ; Vo, Camilla Trang ; Kambe, Yuki ; Wang, Xinxing ; Beinlich, Felix R.M. ; Lee, Ashley Bomin ; Ram, Tina R. ; Wang, Fangying ; Gorzo, Kelsea A. ; Kamijo, Yuki ; Boisvert, Marc ; Nishinami, Suguru ; Kawamura, Genki ; Ozawa, Takeaki ; Toda, Hirofumi ; Gordon, Grant R. ; Ge, Shaoyu ; Hirase, Hajime ; Nedergaard, Maiken ; Paquet, Marie Eve ; Drobizhev, Mikhail ; Podgorski, Kaspar ; Campbell, Robert E. / Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. In: Nature Communications. 2023 ; Vol. 14, No. 1.

Bibtex

@article{c27da587574542c0b45423131cb4ace5,

title = "Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging",

abstract = "l-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- and intra-cellular dynamics of l-lactate are currently hampered by the limited selection and performance of l-lactate-specific genetically encoded biosensors. Here we now report a spectrally and functionally orthogonal pair of high-performance genetically encoded biosensors: a green fluorescent extracellular l-lactate biosensor, designated eLACCO2.1, and a red fluorescent intracellular l-lactate biosensor, designated R-iLACCO1. eLACCO2.1 exhibits excellent membrane localization and robust fluorescence response. To the best of our knowledge, R-iLACCO1 and its affinity variants exhibit larger fluorescence responses than any previously reported intracellular l-lactate biosensor. We demonstrate spectrally and spatially multiplexed imaging of l-lactate dynamics by coexpression of eLACCO2.1 and R-iLACCO1 in cultured cells, and in vivo imaging of extracellular and intracellular l-lactate dynamics in mice.",

author = "Yusuke Nasu and Abhi Aggarwal and Le, {Giang N.T.} and Vo, {Camilla Trang} and Yuki Kambe and Xinxing Wang and Beinlich, {Felix R.M.} and Lee, {Ashley Bomin} and Ram, {Tina R.} and Fangying Wang and Gorzo, {Kelsea A.} and Yuki Kamijo and Marc Boisvert and Suguru Nishinami and Genki Kawamura and Takeaki Ozawa and Hirofumi Toda and Gordon, {Grant R.} and Shaoyu Ge and Hajime Hirase and Maiken Nedergaard and Paquet, {Marie Eve} and Mikhail Drobizhev and Kaspar Podgorski and Campbell, {Robert E.}",

note = "Publisher Copyright: {\textcopyright} 2023, Springer Nature Limited.",

year = "2023",

doi = "10.1038/s41467-023-42230-5",

language = "English",

volume = "14",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging

AU - Nasu, Yusuke

AU - Aggarwal, Abhi

AU - Le, Giang N.T.

AU - Vo, Camilla Trang

AU - Kambe, Yuki

AU - Wang, Xinxing

AU - Beinlich, Felix R.M.

AU - Lee, Ashley Bomin

AU - Ram, Tina R.

AU - Wang, Fangying

AU - Gorzo, Kelsea A.

AU - Kamijo, Yuki

AU - Boisvert, Marc

AU - Nishinami, Suguru

AU - Kawamura, Genki

AU - Ozawa, Takeaki

AU - Toda, Hirofumi

AU - Gordon, Grant R.

AU - Ge, Shaoyu

AU - Hirase, Hajime

AU - Nedergaard, Maiken

AU - Paquet, Marie Eve

AU - Drobizhev, Mikhail

AU - Podgorski, Kaspar

AU - Campbell, Robert E.

PY - 2023

Y1 - 2023

N2 - l-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- and intra-cellular dynamics of l-lactate are currently hampered by the limited selection and performance of l-lactate-specific genetically encoded biosensors. Here we now report a spectrally and functionally orthogonal pair of high-performance genetically encoded biosensors: a green fluorescent extracellular l-lactate biosensor, designated eLACCO2.1, and a red fluorescent intracellular l-lactate biosensor, designated R-iLACCO1. eLACCO2.1 exhibits excellent membrane localization and robust fluorescence response. To the best of our knowledge, R-iLACCO1 and its affinity variants exhibit larger fluorescence responses than any previously reported intracellular l-lactate biosensor. We demonstrate spectrally and spatially multiplexed imaging of l-lactate dynamics by coexpression of eLACCO2.1 and R-iLACCO1 in cultured cells, and in vivo imaging of extracellular and intracellular l-lactate dynamics in mice.

AB - l-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- and intra-cellular dynamics of l-lactate are currently hampered by the limited selection and performance of l-lactate-specific genetically encoded biosensors. Here we now report a spectrally and functionally orthogonal pair of high-performance genetically encoded biosensors: a green fluorescent extracellular l-lactate biosensor, designated eLACCO2.1, and a red fluorescent intracellular l-lactate biosensor, designated R-iLACCO1. eLACCO2.1 exhibits excellent membrane localization and robust fluorescence response. To the best of our knowledge, R-iLACCO1 and its affinity variants exhibit larger fluorescence responses than any previously reported intracellular l-lactate biosensor. We demonstrate spectrally and spatially multiplexed imaging of l-lactate dynamics by coexpression of eLACCO2.1 and R-iLACCO1 in cultured cells, and in vivo imaging of extracellular and intracellular l-lactate dynamics in mice.

U2 - 10.1038/s41467-023-42230-5

DO - 10.1038/s41467-023-42230-5

M3 - Journal article

C2 - 37891202

AN - SCOPUS:85175094806

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 6598

ER -

ID: 371929729

Center for Translational Neuromedicine