Glial progenitor cells of the adult human white and grey matter are contextually distinct

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Glial progenitor cells of the adult human white and grey matter are contextually distinct. / Osorio, Maria Joana; Mariani, John N.; Zou, Lisa; Schanz, Steven J.; Heffernan, Kate; Cornwell, Adam; Goldman, Steven A.

In: Glia, Vol. 71, No. 3, 2023, p. 524-540.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Osorio, MJ, Mariani, JN, Zou, L, Schanz, SJ, Heffernan, K, Cornwell, A & Goldman, SA 2023, 'Glial progenitor cells of the adult human white and grey matter are contextually distinct', Glia, vol. 71, no. 3, pp. 524-540. https://doi.org/10.1002/glia.24291

APA

Osorio, M. J., Mariani, J. N., Zou, L., Schanz, S. J., Heffernan, K., Cornwell, A., & Goldman, S. A. (2023). Glial progenitor cells of the adult human white and grey matter are contextually distinct. Glia, 71(3), 524-540. https://doi.org/10.1002/glia.24291

Vancouver

Osorio MJ, Mariani JN, Zou L, Schanz SJ, Heffernan K, Cornwell A et al. Glial progenitor cells of the adult human white and grey matter are contextually distinct. Glia. 2023;71(3):524-540. https://doi.org/10.1002/glia.24291

Author

Osorio, Maria Joana ; Mariani, John N. ; Zou, Lisa ; Schanz, Steven J. ; Heffernan, Kate ; Cornwell, Adam ; Goldman, Steven A. / Glial progenitor cells of the adult human white and grey matter are contextually distinct. In: Glia. 2023 ; Vol. 71, No. 3. pp. 524-540.

Bibtex

@article{0b51c3e1d81246938acb3b3208f510b9,

title = "Glial progenitor cells of the adult human white and grey matter are contextually distinct",

abstract = "Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2-defined hGPCs derived from adult human neocortex and white matter differed in their expression of genes involved in Wnt, NOTCH, BMP and TGFβ signaling, suggesting compartment-selective biases in fate and self-renewal. White matter hGPCs over-expressed the BMP antagonists BAMBI and CHRDL1, suggesting their tonic suppression of astrocytic fate relative to cortical hGPCs, whose relative enrichment of cytoskeletal genes presaged their greater morphological complexity. In human glial chimeric mice, cortical hGPCs assumed larger and more complex morphologies than white matter hGPCs, and both were more complex than their mouse counterparts. These findings suggest that human grey and white matter GPCs comprise context-specific pools with distinct functional biases.",

keywords = "glial heterogeneity, human glial progenitor cells, NG2, white and grey matter",

author = "Osorio, {Maria Joana} and Mariani, {John N.} and Lisa Zou and Schanz, {Steven J.} and Kate Heffernan and Adam Cornwell and Goldman, {Steven A.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. GLIA published by Wiley Periodicals LLC.",

year = "2023",

doi = "10.1002/glia.24291",

language = "English",

volume = "71",

pages = "524--540",

journal = "GLIA",

issn = "0894-1491",

publisher = "JohnWiley & Sons, Inc.",

number = "3",

}

RIS

TY - JOUR

T1 - Glial progenitor cells of the adult human white and grey matter are contextually distinct

AU - Osorio, Maria Joana

AU - Mariani, John N.

AU - Zou, Lisa

AU - Schanz, Steven J.

AU - Heffernan, Kate

AU - Cornwell, Adam

AU - Goldman, Steven A.

PY - 2023

Y1 - 2023

N2 - Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2-defined hGPCs derived from adult human neocortex and white matter differed in their expression of genes involved in Wnt, NOTCH, BMP and TGFβ signaling, suggesting compartment-selective biases in fate and self-renewal. White matter hGPCs over-expressed the BMP antagonists BAMBI and CHRDL1, suggesting their tonic suppression of astrocytic fate relative to cortical hGPCs, whose relative enrichment of cytoskeletal genes presaged their greater morphological complexity. In human glial chimeric mice, cortical hGPCs assumed larger and more complex morphologies than white matter hGPCs, and both were more complex than their mouse counterparts. These findings suggest that human grey and white matter GPCs comprise context-specific pools with distinct functional biases.

AB - Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2-defined hGPCs derived from adult human neocortex and white matter differed in their expression of genes involved in Wnt, NOTCH, BMP and TGFβ signaling, suggesting compartment-selective biases in fate and self-renewal. White matter hGPCs over-expressed the BMP antagonists BAMBI and CHRDL1, suggesting their tonic suppression of astrocytic fate relative to cortical hGPCs, whose relative enrichment of cytoskeletal genes presaged their greater morphological complexity. In human glial chimeric mice, cortical hGPCs assumed larger and more complex morphologies than white matter hGPCs, and both were more complex than their mouse counterparts. These findings suggest that human grey and white matter GPCs comprise context-specific pools with distinct functional biases.

KW - glial heterogeneity

KW - human glial progenitor cells

KW - NG2

KW - white and grey matter

U2 - 10.1002/glia.24291

DO - 10.1002/glia.24291

M3 - Journal article

C2 - 36334067

AN - SCOPUS:85141511055

VL - 71

SP - 524

EP - 540

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 3

ER -

ID: 344367366

Center for Translational Neuromedicine