Flow of cerebrospinal fluid is driven by arterial pulsations and is reduced in hypertension
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Flow of cerebrospinal fluid is driven by arterial pulsations and is reduced in hypertension. / Mestre, Humberto; Tithof, Jeffrey; Du, Ting; Song, Wei; Peng, Weiguo; Sweeney, Amanda M.; Olveda, Genaro; Thomas, John H.; Nedergaard, Maiken; Kelley, Douglas H.
In: Nature Communications, Vol. 9, No. 1, 4878, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Flow of cerebrospinal fluid is driven by arterial pulsations and is reduced in hypertension
AU - Mestre, Humberto
AU - Tithof, Jeffrey
AU - Du, Ting
AU - Song, Wei
AU - Peng, Weiguo
AU - Sweeney, Amanda M.
AU - Olveda, Genaro
AU - Thomas, John H.
AU - Nedergaard, Maiken
AU - Kelley, Douglas H.
PY - 2018
Y1 - 2018
N2 - Flow of cerebrospinal fluid (CSF) through perivascular spaces (PVSs) in the brain is important for clearance of metabolic waste. Arterial pulsations are thought to drive flow, but this has never been quantitatively shown. We used particle tracking to quantify CSF flow velocities in PVSs of live mice. CSF flow is pulsatile and driven primarily by the cardiac cycle. The speed of the arterial wall matches that of the CSF, suggesting arterial wall motion is the principal driving mechanism, via a process known as perivascular pumping. Increasing blood pressure leaves the artery diameter unchanged but changes the pulsations of the arterial wall, increasing backflow and thereby reducing net flow in the PVS. Perfusion-fixation alters the normal flow direction and causes a 10-fold reduction in PVS size. We conclude that particle tracking velocimetry enables the study of CSF flow in unprecedented detail and that studying the PVS in vivo avoids fixation artifacts.
AB - Flow of cerebrospinal fluid (CSF) through perivascular spaces (PVSs) in the brain is important for clearance of metabolic waste. Arterial pulsations are thought to drive flow, but this has never been quantitatively shown. We used particle tracking to quantify CSF flow velocities in PVSs of live mice. CSF flow is pulsatile and driven primarily by the cardiac cycle. The speed of the arterial wall matches that of the CSF, suggesting arterial wall motion is the principal driving mechanism, via a process known as perivascular pumping. Increasing blood pressure leaves the artery diameter unchanged but changes the pulsations of the arterial wall, increasing backflow and thereby reducing net flow in the PVS. Perfusion-fixation alters the normal flow direction and causes a 10-fold reduction in PVS size. We conclude that particle tracking velocimetry enables the study of CSF flow in unprecedented detail and that studying the PVS in vivo avoids fixation artifacts.
U2 - 10.1038/s41467-018-07318-3
DO - 10.1038/s41467-018-07318-3
M3 - Journal article
C2 - 30451853
AN - SCOPUS:85056707037
VL - 9
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4878
ER -
ID: 209800922