Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism
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Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism. / Lundgaard, Iben; Li, Baoman; Xie, Lulu; Kang, Hongyi; Sanggaard, Simon; Haswell, John D R; Sun, Wei; Goldman, Siri; Blekot, Solomiya; Nielsen, Michael; Takano, Takahiro; Deane, Rashid; Nedergaard, Maiken.
In: Nature Communications, Vol. 6, 6807, 2015.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism
AU - Lundgaard, Iben
AU - Li, Baoman
AU - Xie, Lulu
AU - Kang, Hongyi
AU - Sanggaard, Simon
AU - Haswell, John D R
AU - Sun, Wei
AU - Goldman, Siri
AU - Blekot, Solomiya
AU - Nielsen, Michael
AU - Takano, Takahiro
AU - Deane, Rashid
AU - Nedergaard, Maiken
PY - 2015
Y1 - 2015
N2 - Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identify the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.
AB - Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identify the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.
U2 - 10.1038/ncomms7807
DO - 10.1038/ncomms7807
M3 - Journal article
C2 - 25904018
VL - 6
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 6807
ER -
ID: 152955979