Direct Measurement of Cerebrospinal Fluid Production in Mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Direct Measurement of Cerebrospinal Fluid Production in Mice. / Liu, Guojun; Mestre, Humberto; Sweeney, Amanda M.; Sun, Qian; Weikop, Pia; Du, Ting; Nedergaard, Maiken.

In: Cell Reports, Vol. 33, No. 12, 108524, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Liu, G, Mestre, H, Sweeney, AM, Sun, Q, Weikop, P, Du, T & Nedergaard, M 2020, 'Direct Measurement of Cerebrospinal Fluid Production in Mice', Cell Reports, vol. 33, no. 12, 108524. https://doi.org/10.1016/j.celrep.2020.108524

APA

Liu, G., Mestre, H., Sweeney, A. M., Sun, Q., Weikop, P., Du, T., & Nedergaard, M. (2020). Direct Measurement of Cerebrospinal Fluid Production in Mice. Cell Reports, 33(12), [108524]. https://doi.org/10.1016/j.celrep.2020.108524

Vancouver

Liu G, Mestre H, Sweeney AM, Sun Q, Weikop P, Du T et al. Direct Measurement of Cerebrospinal Fluid Production in Mice. Cell Reports. 2020;33(12). 108524. https://doi.org/10.1016/j.celrep.2020.108524

Author

Liu, Guojun ; Mestre, Humberto ; Sweeney, Amanda M. ; Sun, Qian ; Weikop, Pia ; Du, Ting ; Nedergaard, Maiken. / Direct Measurement of Cerebrospinal Fluid Production in Mice. In: Cell Reports. 2020 ; Vol. 33, No. 12.

Bibtex

@article{e66b5538c53240a3a652121e97da837d,
title = "Direct Measurement of Cerebrospinal Fluid Production in Mice",
abstract = "The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.",
keywords = "aging, amyloid-β, glymphatic system, sex difference, sleep-wake cycle",
author = "Guojun Liu and Humberto Mestre and Sweeney, {Amanda M.} and Qian Sun and Pia Weikop and Ting Du and Maiken Nedergaard",
year = "2020",
doi = "10.1016/j.celrep.2020.108524",
language = "English",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "12",

}

RIS

TY - JOUR

T1 - Direct Measurement of Cerebrospinal Fluid Production in Mice

AU - Liu, Guojun

AU - Mestre, Humberto

AU - Sweeney, Amanda M.

AU - Sun, Qian

AU - Weikop, Pia

AU - Du, Ting

AU - Nedergaard, Maiken

PY - 2020

Y1 - 2020

N2 - The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.

AB - The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.

KW - aging

KW - amyloid-β

KW - glymphatic system

KW - sex difference

KW - sleep-wake cycle

U2 - 10.1016/j.celrep.2020.108524

DO - 10.1016/j.celrep.2020.108524

M3 - Journal article

C2 - 33357428

AN - SCOPUS:85098106366

VL - 33

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 12

M1 - 108524

ER -

ID: 255098644