Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes

Research output: Contribution to journal › Journal article › Research › peer-review

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Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes. / Li, Jiwen; Pan, Lin; Godoy, Marlesa; Pembroke, William G.; Rexach, Jessica E.; Condro, Michael C.; Alvarado, Alvaro G.; Harteni, Mineli; Chen, Yen-Wei; Stiles, Linsey; Chen, Angela Y.; Wanner, Ina B.; Yang, Xia; Goldman, Steven A.; Geschwind, Daniel H.; Kornblum, Harley; Zhang, Ye.

In: Nature Communications, Vol. 12, No. 1, 3958, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Li, J, Pan, L, Godoy, M, Pembroke, WG, Rexach, JE, Condro, MC, Alvarado, AG, Harteni, M, Chen, Y-W, Stiles, L, Chen, AY, Wanner, IB, Yang, X, Goldman, SA, Geschwind, DH, Kornblum, H & Zhang, Y 2021, 'Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes', Nature Communications, vol. 12, no. 1, 3958. https://doi.org/10.1038/s41467-021-24232-3

APA

Li, J., Pan, L., Godoy, M., Pembroke, W. G., Rexach, J. E., Condro, M. C., Alvarado, A. G., Harteni, M., Chen, Y-W., Stiles, L., Chen, A. Y., Wanner, I. B., Yang, X., Goldman, S. A., Geschwind, D. H., Kornblum, H., & Zhang, Y. (2021). Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes. Nature Communications, 12(1), [3958]. https://doi.org/10.1038/s41467-021-24232-3

Vancouver

Li J, Pan L, Godoy M, Pembroke WG, Rexach JE, Condro MC et al. Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes. Nature Communications. 2021;12(1). 3958. https://doi.org/10.1038/s41467-021-24232-3

Author

Li, Jiwen ; Pan, Lin ; Godoy, Marlesa ; Pembroke, William G. ; Rexach, Jessica E. ; Condro, Michael C. ; Alvarado, Alvaro G. ; Harteni, Mineli ; Chen, Yen-Wei ; Stiles, Linsey ; Chen, Angela Y. ; Wanner, Ina B. ; Yang, Xia ; Goldman, Steven A. ; Geschwind, Daniel H. ; Kornblum, Harley ; Zhang, Ye. / Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes. In: Nature Communications. 2021 ; Vol. 12, No. 1.

Bibtex

@article{ecafdef5e42d47e999931f637854491e,
title = "Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes",
abstract = "Astrocytes are important players in brain development, homeostasis, and disease. Here, the authors compare the transcriptional profiles of human and mouse astrocytes. They report species-specific susceptibility to oxidative stress and response to hypoxic and inflammatory conditions.Astrocytes play important roles in neurological disorders such as stroke, injury, and neurodegeneration. Most knowledge on astrocyte biology is based on studies of mouse models and the similarities and differences between human and mouse astrocytes are insufficiently characterized, presenting a barrier in translational research. Based on analyses of acutely purified astrocytes, serum-free cultures of primary astrocytes, and xenografted chimeric mice, we find extensive conservation in astrocytic gene expression between human and mouse samples. However, the genes involved in defense response and metabolism show species-specific differences. Human astrocytes exhibit greater susceptibility to oxidative stress than mouse astrocytes, due to differences in mitochondrial physiology and detoxification pathways. In addition, we find that mouse but not human astrocytes activate a molecular program for neural repair under hypoxia, whereas human but not mouse astrocytes activate the antigen presentation pathway under inflammatory conditions. Here, we show species-dependent properties of astrocytes, which can be informative for improving translation from mouse models to humans.",
keywords = "GLIAL PROGENITOR CELLS, HUMAN-BRAIN, OXIDATIVE STRESS, RADIAL GLIA, RNA-SEQ, TRANSCRIPTOME, MICE, DISEASE, MODELS, PURIFICATION",
author = "Jiwen Li and Lin Pan and Marlesa Godoy and Pembroke, {William G.} and Rexach, {Jessica E.} and Condro, {Michael C.} and Alvarado, {Alvaro G.} and Mineli Harteni and Yen-Wei Chen and Linsey Stiles and Chen, {Angela Y.} and Wanner, {Ina B.} and Xia Yang and Goldman, {Steven A.} and Geschwind, {Daniel H.} and Harley Kornblum and Ye Zhang",
year = "2021",
doi = "10.1038/s41467-021-24232-3",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes

AU - Li, Jiwen

AU - Pan, Lin

AU - Godoy, Marlesa

AU - Pembroke, William G.

AU - Rexach, Jessica E.

AU - Condro, Michael C.

AU - Alvarado, Alvaro G.

AU - Harteni, Mineli

AU - Chen, Yen-Wei

AU - Stiles, Linsey

AU - Chen, Angela Y.

AU - Wanner, Ina B.

AU - Yang, Xia

AU - Goldman, Steven A.

AU - Geschwind, Daniel H.

AU - Kornblum, Harley

AU - Zhang, Ye

PY - 2021

Y1 - 2021

N2 - Astrocytes are important players in brain development, homeostasis, and disease. Here, the authors compare the transcriptional profiles of human and mouse astrocytes. They report species-specific susceptibility to oxidative stress and response to hypoxic and inflammatory conditions.Astrocytes play important roles in neurological disorders such as stroke, injury, and neurodegeneration. Most knowledge on astrocyte biology is based on studies of mouse models and the similarities and differences between human and mouse astrocytes are insufficiently characterized, presenting a barrier in translational research. Based on analyses of acutely purified astrocytes, serum-free cultures of primary astrocytes, and xenografted chimeric mice, we find extensive conservation in astrocytic gene expression between human and mouse samples. However, the genes involved in defense response and metabolism show species-specific differences. Human astrocytes exhibit greater susceptibility to oxidative stress than mouse astrocytes, due to differences in mitochondrial physiology and detoxification pathways. In addition, we find that mouse but not human astrocytes activate a molecular program for neural repair under hypoxia, whereas human but not mouse astrocytes activate the antigen presentation pathway under inflammatory conditions. Here, we show species-dependent properties of astrocytes, which can be informative for improving translation from mouse models to humans.

AB - Astrocytes are important players in brain development, homeostasis, and disease. Here, the authors compare the transcriptional profiles of human and mouse astrocytes. They report species-specific susceptibility to oxidative stress and response to hypoxic and inflammatory conditions.Astrocytes play important roles in neurological disorders such as stroke, injury, and neurodegeneration. Most knowledge on astrocyte biology is based on studies of mouse models and the similarities and differences between human and mouse astrocytes are insufficiently characterized, presenting a barrier in translational research. Based on analyses of acutely purified astrocytes, serum-free cultures of primary astrocytes, and xenografted chimeric mice, we find extensive conservation in astrocytic gene expression between human and mouse samples. However, the genes involved in defense response and metabolism show species-specific differences. Human astrocytes exhibit greater susceptibility to oxidative stress than mouse astrocytes, due to differences in mitochondrial physiology and detoxification pathways. In addition, we find that mouse but not human astrocytes activate a molecular program for neural repair under hypoxia, whereas human but not mouse astrocytes activate the antigen presentation pathway under inflammatory conditions. Here, we show species-dependent properties of astrocytes, which can be informative for improving translation from mouse models to humans.

KW - GLIAL PROGENITOR CELLS

KW - HUMAN-BRAIN

KW - OXIDATIVE STRESS

KW - RADIAL GLIA

KW - RNA-SEQ

KW - TRANSCRIPTOME

KW - MICE

KW - DISEASE

KW - MODELS

KW - PURIFICATION

U2 - 10.1038/s41467-021-24232-3

DO - 10.1038/s41467-021-24232-3

M3 - Journal article

C2 - 34172753

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3958

ER -

ID: 277647227