Cisterna magna injection in rats to study glymphatic function

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Cisterna magna injection in rats to study glymphatic function. / Ramos, Marta; Burdon Bechet, Nicholas; Battistella, Roberta; Pavan, Chiara; Xavier, Anna L.R.; Nedergaard, Maiken; Lundgaard, Iben.

Astrocytes: Methods and Protocols. ed. / Barbara Di Benedetto. Humana Press, 2019. p. 97-104 (Methods in Molecular Biology, Vol. 1938).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Ramos, M, Burdon Bechet, N, Battistella, R, Pavan, C, Xavier, ALR, Nedergaard, M & Lundgaard, I 2019, Cisterna magna injection in rats to study glymphatic function. in B Di Benedetto (ed.), Astrocytes: Methods and Protocols. Humana Press, Methods in Molecular Biology, vol. 1938, pp. 97-104. https://doi.org/10.1007/978-1-4939-9068-9_7

APA

Ramos, M., Burdon Bechet, N., Battistella, R., Pavan, C., Xavier, A. L. R., Nedergaard, M., & Lundgaard, I. (2019). Cisterna magna injection in rats to study glymphatic function. In B. Di Benedetto (Ed.), Astrocytes: Methods and Protocols (pp. 97-104). Humana Press. Methods in Molecular Biology Vol. 1938 https://doi.org/10.1007/978-1-4939-9068-9_7

Vancouver

Ramos M, Burdon Bechet N, Battistella R, Pavan C, Xavier ALR, Nedergaard M et al. Cisterna magna injection in rats to study glymphatic function. In Di Benedetto B, editor, Astrocytes: Methods and Protocols. Humana Press. 2019. p. 97-104. (Methods in Molecular Biology, Vol. 1938). https://doi.org/10.1007/978-1-4939-9068-9_7

Author

Ramos, Marta ; Burdon Bechet, Nicholas ; Battistella, Roberta ; Pavan, Chiara ; Xavier, Anna L.R. ; Nedergaard, Maiken ; Lundgaard, Iben. / Cisterna magna injection in rats to study glymphatic function. Astrocytes: Methods and Protocols. editor / Barbara Di Benedetto. Humana Press, 2019. pp. 97-104 (Methods in Molecular Biology, Vol. 1938).

Bibtex

@inbook{134760cbf60a4cdc9881b2c78e4f2878,
title = "Cisterna magna injection in rats to study glymphatic function",
abstract = "The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.",
keywords = "Cerebrospinal fluid, Cisterna magna injection, Glymphatic system, Tracer",
author = "Marta Ramos and {Burdon Bechet}, Nicholas and Roberta Battistella and Chiara Pavan and Xavier, {Anna L.R.} and Maiken Nedergaard and Iben Lundgaard",
year = "2019",
doi = "10.1007/978-1-4939-9068-9_7",
language = "English",
isbn = "978-1-4939-9067-2",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "97--104",
editor = "{Di Benedetto}, Barbara",
booktitle = "Astrocytes",
address = "United States",

}

RIS

TY - CHAP

T1 - Cisterna magna injection in rats to study glymphatic function

AU - Ramos, Marta

AU - Burdon Bechet, Nicholas

AU - Battistella, Roberta

AU - Pavan, Chiara

AU - Xavier, Anna L.R.

AU - Nedergaard, Maiken

AU - Lundgaard, Iben

PY - 2019

Y1 - 2019

N2 - The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.

AB - The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.

KW - Cerebrospinal fluid

KW - Cisterna magna injection

KW - Glymphatic system

KW - Tracer

U2 - 10.1007/978-1-4939-9068-9_7

DO - 10.1007/978-1-4939-9068-9_7

M3 - Book chapter

C2 - 30617975

AN - SCOPUS:85059928469

SN - 978-1-4939-9067-2

T3 - Methods in Molecular Biology

SP - 97

EP - 104

BT - Astrocytes

A2 - Di Benedetto, Barbara

PB - Humana Press

ER -

ID: 226256796