Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection. / Raghunandan, Aditya; Ladron-De-guevara, Antonio; Tithof, Jeffrey; Mestre, Humberto; Du, Ting; Nedergaard, Maiken; Thomas, John H.; Kelley, Douglas H.

In: eLife, Vol. 10, e65958, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Raghunandan, A, Ladron-De-guevara, A, Tithof, J, Mestre, H, Du, T, Nedergaard, M, Thomas, JH & Kelley, DH 2021, 'Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection', eLife, vol. 10, e65958. https://doi.org/10.7554/eLife.65958

APA

Raghunandan, A., Ladron-De-guevara, A., Tithof, J., Mestre, H., Du, T., Nedergaard, M., Thomas, J. H., & Kelley, D. H. (2021). Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection. eLife, 10, [e65958]. https://doi.org/10.7554/eLife.65958

Vancouver

Raghunandan A, Ladron-De-guevara A, Tithof J, Mestre H, Du T, Nedergaard M et al. Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection. eLife. 2021;10. e65958. https://doi.org/10.7554/eLife.65958

Author

Raghunandan, Aditya ; Ladron-De-guevara, Antonio ; Tithof, Jeffrey ; Mestre, Humberto ; Du, Ting ; Nedergaard, Maiken ; Thomas, John H. ; Kelley, Douglas H. / Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection. In: eLife. 2021 ; Vol. 10.

Bibtex

@article{d73807f268784182bb1813d70928f919,
title = "Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection",
abstract = "Cerebrospinal fluid (CSF) flowing through periarterial spaces is integral to the brain{\textquoteright}s mechanism for clearing metabolic waste products. Experiments that track tracer particles injected into the cisterna magna (CM) of mouse brains have shown evidence of pulsatile CSF flow in perivascular spaces surrounding pial arteries, with a bulk flow in the same direction as blood flow. However, the driving mechanism remains elusive. Several studies have suggested that the bulk flow might be an artifact, driven by the injection itself. Here, we address this hypothesis with new in vivo experiments where tracer particles are injected into the CM using a dual-syringe system, with simultaneous injection and withdrawal of equal amounts of fluid. This method produces no net increase in CSF volume and no significant increase in intracranial pressure. Yet, particle-tracking reveals flows that are consistent in all respects with the flows observed in earlier experiments with single-syringe injection.",
author = "Aditya Raghunandan and Antonio Ladron-De-guevara and Jeffrey Tithof and Humberto Mestre and Ting Du and Maiken Nedergaard and Thomas, {John H.} and Kelley, {Douglas H.}",
year = "2021",
doi = "10.7554/eLife.65958",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection

AU - Raghunandan, Aditya

AU - Ladron-De-guevara, Antonio

AU - Tithof, Jeffrey

AU - Mestre, Humberto

AU - Du, Ting

AU - Nedergaard, Maiken

AU - Thomas, John H.

AU - Kelley, Douglas H.

PY - 2021

Y1 - 2021

N2 - Cerebrospinal fluid (CSF) flowing through periarterial spaces is integral to the brain’s mechanism for clearing metabolic waste products. Experiments that track tracer particles injected into the cisterna magna (CM) of mouse brains have shown evidence of pulsatile CSF flow in perivascular spaces surrounding pial arteries, with a bulk flow in the same direction as blood flow. However, the driving mechanism remains elusive. Several studies have suggested that the bulk flow might be an artifact, driven by the injection itself. Here, we address this hypothesis with new in vivo experiments where tracer particles are injected into the CM using a dual-syringe system, with simultaneous injection and withdrawal of equal amounts of fluid. This method produces no net increase in CSF volume and no significant increase in intracranial pressure. Yet, particle-tracking reveals flows that are consistent in all respects with the flows observed in earlier experiments with single-syringe injection.

AB - Cerebrospinal fluid (CSF) flowing through periarterial spaces is integral to the brain’s mechanism for clearing metabolic waste products. Experiments that track tracer particles injected into the cisterna magna (CM) of mouse brains have shown evidence of pulsatile CSF flow in perivascular spaces surrounding pial arteries, with a bulk flow in the same direction as blood flow. However, the driving mechanism remains elusive. Several studies have suggested that the bulk flow might be an artifact, driven by the injection itself. Here, we address this hypothesis with new in vivo experiments where tracer particles are injected into the CM using a dual-syringe system, with simultaneous injection and withdrawal of equal amounts of fluid. This method produces no net increase in CSF volume and no significant increase in intracranial pressure. Yet, particle-tracking reveals flows that are consistent in all respects with the flows observed in earlier experiments with single-syringe injection.

U2 - 10.7554/eLife.65958

DO - 10.7554/eLife.65958

M3 - Journal article

C2 - 33687330

AN - SCOPUS:85103306176

VL - 10

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e65958

ER -

ID: 259836355