Anxiety control by astrocytes in the lateral habenula
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Anxiety control by astrocytes in the lateral habenula. / Tan, Wanqin; Ikoma, Yoko; Takahashi, Yusuke; Konno, Ayumu; Hirai, Hirokazu; Hirase, Hajime; Matsui, Ko.
In: Neuroscience Research, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Anxiety control by astrocytes in the lateral habenula
AU - Tan, Wanqin
AU - Ikoma, Yoko
AU - Takahashi, Yusuke
AU - Konno, Ayumu
AU - Hirai, Hirokazu
AU - Hirase, Hajime
AU - Matsui, Ko
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - The potential role of astrocytes in lateral habenula (LHb) in modulating anxiety was explored in this study. The habenula are a pair of small nuclei located above the thalamus, known for their involvement in punishment avoidance and anxiety. Herein, we observed an increase in theta-band oscillations of local field potentials (LFPs) in the LHb when mice were exposed to anxiety-inducing environments. Electrical stimulation of LHb at theta-band frequency promoted anxiety-like behavior. Calcium (Ca2+) levels and pH in the cytosol of astrocytes and local brain blood volume changes were studied in mice expressing either a Ca2+ or a pH sensor protein specifically in astrocytes and mScarlet fluorescent protein in the blood plasma using fiber photometry. An acidification response to anxiety was observed. Photoactivation of archaerhopsin-T (ArchT), an optogenetic tool that acts as an outward proton pump, results in intracellular alkalinization. Photostimulation of LHb in astrocyte-specific ArchT-expressing mice resulted in dissipation of theta-band LFP oscillation in an anxiogenic environment and suppression of anxiety-like behavior. These findings provide evidence that LHb astrocytes modulate anxiety and may offer a new target for treatment of anxiety disorders.
AB - The potential role of astrocytes in lateral habenula (LHb) in modulating anxiety was explored in this study. The habenula are a pair of small nuclei located above the thalamus, known for their involvement in punishment avoidance and anxiety. Herein, we observed an increase in theta-band oscillations of local field potentials (LFPs) in the LHb when mice were exposed to anxiety-inducing environments. Electrical stimulation of LHb at theta-band frequency promoted anxiety-like behavior. Calcium (Ca2+) levels and pH in the cytosol of astrocytes and local brain blood volume changes were studied in mice expressing either a Ca2+ or a pH sensor protein specifically in astrocytes and mScarlet fluorescent protein in the blood plasma using fiber photometry. An acidification response to anxiety was observed. Photoactivation of archaerhopsin-T (ArchT), an optogenetic tool that acts as an outward proton pump, results in intracellular alkalinization. Photostimulation of LHb in astrocyte-specific ArchT-expressing mice resulted in dissipation of theta-band LFP oscillation in an anxiogenic environment and suppression of anxiety-like behavior. These findings provide evidence that LHb astrocytes modulate anxiety and may offer a new target for treatment of anxiety disorders.
KW - Anxiety
KW - Astrocyte
KW - Cytosolic pH
KW - Fiber photometry
KW - Habenula
KW - Local brain environment
KW - Optogenetics
KW - Theta band LFP
U2 - 10.1016/j.neures.2024.01.006
DO - 10.1016/j.neures.2024.01.006
M3 - Journal article
C2 - 38311032
AN - SCOPUS:85184752108
JO - Neuroscience research. Supplement : the official journal of the Japan Neuroscience Society
JF - Neuroscience research. Supplement : the official journal of the Japan Neuroscience Society
SN - 0921-8696
ER -
ID: 383429736