A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration. / Ghochani, Yasmin; Muthukrishnan, Sree Deepthi; Sohrabi, Alireza; Kawaguchi, Riki; Condro, Michael C.; Bastola, Soniya; Gao, Fuying; Qin, Yue; Mottahedeh, Jack; Iruela-Arispe, M. Luisa; Rao, Nagesh; Laks, Dan R.; Liau, Linda M.; Mathern, Gary W.; Goldman, Steven A.; Carmichael, S. Thomas; Nakano, Ichiro; Coppola, Giovanni; Seidlits, Stephanie K.; Kornblum, Harley I.

In: Cell Reports, Vol. 41, No. 3, 111511, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ghochani, Y, Muthukrishnan, SD, Sohrabi, A, Kawaguchi, R, Condro, MC, Bastola, S, Gao, F, Qin, Y, Mottahedeh, J, Iruela-Arispe, ML, Rao, N, Laks, DR, Liau, LM, Mathern, GW, Goldman, SA, Carmichael, ST, Nakano, I, Coppola, G, Seidlits, SK & Kornblum, HI 2022, 'A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration', Cell Reports, vol. 41, no. 3, 111511. https://doi.org/10.1016/j.celrep.2022.111511

APA

Ghochani, Y., Muthukrishnan, S. D., Sohrabi, A., Kawaguchi, R., Condro, M. C., Bastola, S., Gao, F., Qin, Y., Mottahedeh, J., Iruela-Arispe, M. L., Rao, N., Laks, D. R., Liau, L. M., Mathern, G. W., Goldman, S. A., Carmichael, S. T., Nakano, I., Coppola, G., Seidlits, S. K., & Kornblum, H. I. (2022). A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration. Cell Reports, 41(3), [111511]. https://doi.org/10.1016/j.celrep.2022.111511

Vancouver

Ghochani Y, Muthukrishnan SD, Sohrabi A, Kawaguchi R, Condro MC, Bastola S et al. A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration. Cell Reports. 2022;41(3). 111511. https://doi.org/10.1016/j.celrep.2022.111511

Author

Ghochani, Yasmin ; Muthukrishnan, Sree Deepthi ; Sohrabi, Alireza ; Kawaguchi, Riki ; Condro, Michael C. ; Bastola, Soniya ; Gao, Fuying ; Qin, Yue ; Mottahedeh, Jack ; Iruela-Arispe, M. Luisa ; Rao, Nagesh ; Laks, Dan R. ; Liau, Linda M. ; Mathern, Gary W. ; Goldman, Steven A. ; Carmichael, S. Thomas ; Nakano, Ichiro ; Coppola, Giovanni ; Seidlits, Stephanie K. ; Kornblum, Harley I. / A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration. In: Cell Reports. 2022 ; Vol. 41, No. 3.

Bibtex

@article{d35c2af7e1b444ebbfb149fd7890e00f,
title = "A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration",
abstract = "Glioblastoma (GBM) is characterized by extensive microvascular hyperproliferation. In addition to supplying blood to the tumor, GBM vessels also provide trophic support to glioma cells and serve as conduits for migration into the surrounding brain, promoting recurrence. Here, we enrich CD31-expressing glioma vascular cells (GVCs) and A2B5-expressing glioma tumor cells (GTCs) from primary GBM and use RNA sequencing to create a comprehensive molecular interaction map of the secreted and extracellular factors elaborated by GVCs that can interact with receptors and membrane molecules on GTCs. To validate our findings, we utilize functional assays, including a hydrogel-based migration assay and in vivo mouse models to demonstrate that one identified factor, the little-studied integrin binding sialoprotein (IBSP), enhances tumor growth and promotes the migration of GTCs along the vasculature. This perivascular niche interactome will serve as a resource to the research community in defining the potential functions of the GBM vasculature.",
keywords = "CP: Cancer, endothelial cells, glioblastoma, glioma, hydrogel, IBSP, interactome, migration, pericytes, perivascular niche, stem cell",
author = "Yasmin Ghochani and Muthukrishnan, {Sree Deepthi} and Alireza Sohrabi and Riki Kawaguchi and Condro, {Michael C.} and Soniya Bastola and Fuying Gao and Yue Qin and Jack Mottahedeh and Iruela-Arispe, {M. Luisa} and Nagesh Rao and Laks, {Dan R.} and Liau, {Linda M.} and Mathern, {Gary W.} and Goldman, {Steven A.} and Carmichael, {S. Thomas} and Ichiro Nakano and Giovanni Coppola and Seidlits, {Stephanie K.} and Kornblum, {Harley I.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.celrep.2022.111511",
language = "English",
volume = "41",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration

AU - Ghochani, Yasmin

AU - Muthukrishnan, Sree Deepthi

AU - Sohrabi, Alireza

AU - Kawaguchi, Riki

AU - Condro, Michael C.

AU - Bastola, Soniya

AU - Gao, Fuying

AU - Qin, Yue

AU - Mottahedeh, Jack

AU - Iruela-Arispe, M. Luisa

AU - Rao, Nagesh

AU - Laks, Dan R.

AU - Liau, Linda M.

AU - Mathern, Gary W.

AU - Goldman, Steven A.

AU - Carmichael, S. Thomas

AU - Nakano, Ichiro

AU - Coppola, Giovanni

AU - Seidlits, Stephanie K.

AU - Kornblum, Harley I.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Glioblastoma (GBM) is characterized by extensive microvascular hyperproliferation. In addition to supplying blood to the tumor, GBM vessels also provide trophic support to glioma cells and serve as conduits for migration into the surrounding brain, promoting recurrence. Here, we enrich CD31-expressing glioma vascular cells (GVCs) and A2B5-expressing glioma tumor cells (GTCs) from primary GBM and use RNA sequencing to create a comprehensive molecular interaction map of the secreted and extracellular factors elaborated by GVCs that can interact with receptors and membrane molecules on GTCs. To validate our findings, we utilize functional assays, including a hydrogel-based migration assay and in vivo mouse models to demonstrate that one identified factor, the little-studied integrin binding sialoprotein (IBSP), enhances tumor growth and promotes the migration of GTCs along the vasculature. This perivascular niche interactome will serve as a resource to the research community in defining the potential functions of the GBM vasculature.

AB - Glioblastoma (GBM) is characterized by extensive microvascular hyperproliferation. In addition to supplying blood to the tumor, GBM vessels also provide trophic support to glioma cells and serve as conduits for migration into the surrounding brain, promoting recurrence. Here, we enrich CD31-expressing glioma vascular cells (GVCs) and A2B5-expressing glioma tumor cells (GTCs) from primary GBM and use RNA sequencing to create a comprehensive molecular interaction map of the secreted and extracellular factors elaborated by GVCs that can interact with receptors and membrane molecules on GTCs. To validate our findings, we utilize functional assays, including a hydrogel-based migration assay and in vivo mouse models to demonstrate that one identified factor, the little-studied integrin binding sialoprotein (IBSP), enhances tumor growth and promotes the migration of GTCs along the vasculature. This perivascular niche interactome will serve as a resource to the research community in defining the potential functions of the GBM vasculature.

KW - CP: Cancer

KW - endothelial cells

KW - glioblastoma

KW - glioma

KW - hydrogel

KW - IBSP

KW - interactome

KW - migration

KW - pericytes

KW - perivascular niche

KW - stem cell

U2 - 10.1016/j.celrep.2022.111511

DO - 10.1016/j.celrep.2022.111511

M3 - Journal article

C2 - 36261010

AN - SCOPUS:85140416260

VL - 41

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 3

M1 - 111511

ER -

ID: 343131198