Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation
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Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. / Wang, Xiaowen; Delle, Christine; Asiminas, Antonis; Akther, Sonam; Vittani, Marta; Brøgger, Peter; Kusk, Peter; Vo, Camilla Trang; Radovanovic, Tessa; Konno, Ayumu; Hirai, Hirokazu; Fukuda, Masahiro; Weikop, Pia; Goldman, Steven A; Nedergaard, Maiken; Hirase, Hajime.
In: Cell Reports Methods, Vol. 2, No. 10, 100302, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation
AU - Wang, Xiaowen
AU - Delle, Christine
AU - Asiminas, Antonis
AU - Akther, Sonam
AU - Vittani, Marta
AU - Brøgger, Peter
AU - Kusk, Peter
AU - Vo, Camilla Trang
AU - Radovanovic, Tessa
AU - Konno, Ayumu
AU - Hirai, Hirokazu
AU - Fukuda, Masahiro
AU - Weikop, Pia
AU - Goldman, Steven A
AU - Nedergaard, Maiken
AU - Hirase, Hajime
N1 - © 2022 The Author(s).
PY - 2022
Y1 - 2022
N2 - Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.
AB - Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.
U2 - 10.1016/j.crmeth.2022.100302
DO - 10.1016/j.crmeth.2022.100302
M3 - Journal article
C2 - 36313804
VL - 2
JO - Cell Reports Methods
JF - Cell Reports Methods
SN - 2667-2375
IS - 10
M1 - 100302
ER -
ID: 329708257