Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Number of pages | 41 |
| Volume | 2564 |
| Place of Publication | New York, NY |
| Publisher | Humana Press |
| Publication date | 2023 |
| Pages | 143-183 |
| Chapter | 7 |
| ISBN (Print) | 978-1-0716-2666-5 |
| ISBN (Electronic) | 978-1-0716-2667-2 |
| DOIs | |
| Publication status | Published - 2023 |
| Externally published | Yes |
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2564 |
| ISSN | 1064-3745 |
Bibliographical note
Funding Information:
N.L.B., F.H., G.B., and J.T. contributed equally to the preparation of the chapter. S.N., U.K., T.G., and T.D. are co-corresponding authors. The PS characterization was supported by grants from the Bioeconomy Science Center awarded to F.H. The scientific activities of the Bioeconomy Science Center were financially supported by the Ministry of Innovation, Science and Research of the German federal state of North Rhine-Westphalia MIWF within the frame-work of the NRW Strategieprojekt BioSC (No. 313/323-400-00213). The authors thank Ivan Gushchin for his contributions to writing and editing of this chapter.
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Flavin, Flavin-binding fluorescent protein, Fluorescence brightness, Fluorescence lifetime, Fluorescence microscopy, Genetically encoded photosensitizer, LOV domain, Phototoxicity
Research areas
ID: 340409831