A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. / Wu, Sheng-Yi; Wen, Yurong; Serre, Nelson B C; Laursen, Cathrine Charlotte Heiede; Dietz, Andrea Grostøl; Taylor, Brian R; Drobizhev, Mikhail; Molina, Rosana S; Aggarwal, Abhi; Rancic, Vladimir; Becker, Michael; Ballanyi, Klaus; Podgorski, Kaspar; Hirase, Hajime; Nedergaard, Maiken; Fendrych, Matyáš; Lemieux, M Joanne; Eberl, Daniel F; Kay, Alan R; Campbell, Robert E; Shen, Yi.

In: PLOS Biology, Vol. 20, No. 9, e3001772, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wu, S-Y, Wen, Y, Serre, NBC, Laursen, CCH, Dietz, AG, Taylor, BR, Drobizhev, M, Molina, RS, Aggarwal, A, Rancic, V, Becker, M, Ballanyi, K, Podgorski, K, Hirase, H, Nedergaard, M, Fendrych, M, Lemieux, MJ, Eberl, DF, Kay, AR, Campbell, RE & Shen, Y 2023, 'A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life', PLOS Biology, vol. 20, no. 9, e3001772. https://doi.org/10.1371/journal.pbio.3001772

APA

Wu, S-Y., Wen, Y., Serre, N. B. C., Laursen, C. C. H., Dietz, A. G., Taylor, B. R., Drobizhev, M., Molina, R. S., Aggarwal, A., Rancic, V., Becker, M., Ballanyi, K., Podgorski, K., Hirase, H., Nedergaard, M., Fendrych, M., Lemieux, M. J., Eberl, D. F., Kay, A. R., ... Shen, Y. (2023). A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. PLOS Biology, 20(9), [e3001772]. https://doi.org/10.1371/journal.pbio.3001772

Vancouver

Wu S-Y, Wen Y, Serre NBC, Laursen CCH, Dietz AG, Taylor BR et al. A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. PLOS Biology. 2023;20(9). e3001772. https://doi.org/10.1371/journal.pbio.3001772

Author

Wu, Sheng-Yi ; Wen, Yurong ; Serre, Nelson B C ; Laursen, Cathrine Charlotte Heiede ; Dietz, Andrea Grostøl ; Taylor, Brian R ; Drobizhev, Mikhail ; Molina, Rosana S ; Aggarwal, Abhi ; Rancic, Vladimir ; Becker, Michael ; Ballanyi, Klaus ; Podgorski, Kaspar ; Hirase, Hajime ; Nedergaard, Maiken ; Fendrych, Matyáš ; Lemieux, M Joanne ; Eberl, Daniel F ; Kay, Alan R ; Campbell, Robert E ; Shen, Yi. / A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. In: PLOS Biology. 2023 ; Vol. 20, No. 9.

Bibtex

@article{a8b75c50b0894f09affb793d45254f4f,
title = "A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life",
abstract = "Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.",
keywords = "Animals, Biosensing Techniques/methods, Fluorescence Resonance Energy Transfer/methods, Ions, Mice, Potassium",
author = "Sheng-Yi Wu and Yurong Wen and Serre, {Nelson B C} and Laursen, {Cathrine Charlotte Heiede} and Dietz, {Andrea Grost{\o}l} and Taylor, {Brian R} and Mikhail Drobizhev and Molina, {Rosana S} and Abhi Aggarwal and Vladimir Rancic and Michael Becker and Klaus Ballanyi and Kaspar Podgorski and Hajime Hirase and Maiken Nedergaard and Maty{\'a}{\v s} Fendrych and Lemieux, {M Joanne} and Eberl, {Daniel F} and Kay, {Alan R} and Campbell, {Robert E} and Yi Shen",
year = "2023",
doi = "10.1371/journal.pbio.3001772",
language = "English",
volume = "20",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life

AU - Wu, Sheng-Yi

AU - Wen, Yurong

AU - Serre, Nelson B C

AU - Laursen, Cathrine Charlotte Heiede

AU - Dietz, Andrea Grostøl

AU - Taylor, Brian R

AU - Drobizhev, Mikhail

AU - Molina, Rosana S

AU - Aggarwal, Abhi

AU - Rancic, Vladimir

AU - Becker, Michael

AU - Ballanyi, Klaus

AU - Podgorski, Kaspar

AU - Hirase, Hajime

AU - Nedergaard, Maiken

AU - Fendrych, Matyáš

AU - Lemieux, M Joanne

AU - Eberl, Daniel F

AU - Kay, Alan R

AU - Campbell, Robert E

AU - Shen, Yi

PY - 2023

Y1 - 2023

N2 - Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.

AB - Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.

KW - Animals

KW - Biosensing Techniques/methods

KW - Fluorescence Resonance Energy Transfer/methods

KW - Ions

KW - Mice

KW - Potassium

U2 - 10.1371/journal.pbio.3001772

DO - 10.1371/journal.pbio.3001772

M3 - Journal article

C2 - 36067248

VL - 20

JO - PLoS Biology

JF - PLoS Biology

SN - 1544-9173

IS - 9

M1 - e3001772

ER -

ID: 329708023